Effect of Human Embryonic Stem Cells on Acute Myeloid Leukemia KG-1a Cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 389-394, 2018.
Article
in Zh
| WPRIM
| ID: wpr-690979
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of embryonic stem cells on the proliferation and apoptosis in human acute myeloid leukemia cell line KG-1a and to explore its potential mechanism.</p><p><b>METHODS</b>The direct co-culture system between human embryonic stem cells H9 and human acute myeloid leukemia cell line KG-1a was established, and CCK8 assay was used to detect the proliferation of KG-1a cells. The changes of cell cycle and apoptosis were detected by flow cytometry (FCM). The mRNA expressions of BCL-2, BAX, Caspase-3 were assessed by RT-PCR. Meanwhile, the protein-expressions of BCL-2, BAX, Caspase-3 were detected by Western blot.</p><p><b>RESULTS</b>The proliferation level of KG-1a cells was significantly inhibited by H9, and the apoptotic rate increased, and the cell cycle was blocked at G/M phase. The mRNA-expression and the protein-expression of BAX and Caspase-3 increased, the mRNA and protein-expression of BCL-2 decreased.</p><p><b>CONCLUSION</b>Embryonic stem cells can inhibit the proliferation of KG-1a and induce the apoptosis that maybe relate with the down-regulation of BCL-2 expression and up-regulation of BAX and caspase-3 expression.</p>
Full text:
1
Index:
WPRIM
Main subject:
Leukemia, Myeloid, Acute
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Apoptosis
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Proto-Oncogene Proteins c-bcl-2
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Cell Line, Tumor
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Cell Proliferation
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Bcl-2-Associated X Protein
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Caspase 3
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Human Embryonic Stem Cells
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2018
Type:
Article