Kinetic Parameters of Plasma Albumin Synthesis by Deuterated Water / 肠外与肠内营养
Parenteral & Enteral Nutrition
; (6): 52-55,61, 2018.
Article
in Zh
| WPRIM
| ID: wpr-692113
Responsible library:
WPRO
ABSTRACT
Objective:To measure the kinetic parameters of murine plasma albumin synthesis by deuterated water (2H2O).Methods:Twenty SD rats received 2H2O-labelled intraperitoneal injection and drinking.At the end of the 1st,3rd,5th,6th,10th week,every 4 rats was respectively sacrificed by cardiac puncture for blood collection.Plasma albumin was purified by trichloroacetic acid and ethanol,identified by SDS-PAGE and hydrolyzed by hydrochloric acid.The alanine in albumin and plasma was separated,purified and derived.The deuterium enrichments of alanyl unit in albumin and alanine in plasma were determined by GC-MS.The fractional synthesis rate of albumin and synthetic dynamic equation were calculated.Results:The fractional synthesis rate of plasma albumin was 15.3%/w,and synthetic dynamic equation was ft =1.256 × (1-e-0.153t).Conclusion:The kinetic parameters of plasma albumin synthesis can be measured by 2H2O labeling method,which can be applied in the study of physiological and pathological metabolic mechanism.
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WPRIM
Language:
Zh
Journal:
Parenteral & Enteral Nutrition
Year:
2018
Type:
Article