Study on upconversion nanoparticles-based rapid fluorescence procalcitonin quantitative detection technology / 国际检验医学杂志
International Journal of Laboratory Medicine
;
(12): 67-69,73, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-692633
ABSTRACT
Objective To research and develop the immunochromatographic detection technology of upcon-verting nanoparticles(UCNP)-based rapid fluorescence quantitative procalcitonin (PCT ) detection .Methods UCNP (NaYF4Yb3+ ,Er3+ ) was prepared by solvothermal method ,which was the compound of rare earth yt-terbium and erbium with four fluorine sodium yttrium .UNCP was conducted the modification and silica (SiO2 ) packing by using the reverse microemulsion method .Then the PCT determination method was estab-lished by using the dry-type immunochromatographic technology .Results The water-soluble UCNP with good dispersibility was successfully prepared by using the reverse microemulsion method .The lowest detection limit for detecting PCT by UCNP-based dry-type immunochromatographic technology was 0 .02 ng/mL ,the linear range was 0 .05 -44 .00 ng/mL ,the intra-batch and inter-batch coefficients variable(CV) were <10%and 13% respectively .Conclusion UCNP-based immunochromatography technology is a rapidly and sensitively effective method for quantitatively detecting serum PCT .
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
International Journal of Laboratory Medicine
Year:
2018
Type:
Article
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