High resolution melting analysis for detection of ALDH 2 and ADH1B gene polymorphisms / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12): 1537-1539, 2018.
Article
in Zh
| WPRIM
| ID: wpr-692872
Responsible library:
WPRO
ABSTRACT
Objective To establish the system of high resolution melting for detection of aldehyde dehydro-genase 2(ALDH2) and alcohol dehydrogenase-1B (ADH1B) gene polymorphisms .Methods The short prim-ers were designed for ALDH2 and ADH1B gene .Different PCR products were analyzed using Eva Green dyes after amplification ,which were confirmed by Sanger sequencing .Results The genotypes of ALDH2 rs671 and ADH1B rs1229984 were successfully detected in the same procedure by HRM within 90min ,and the results were consistent with Sanger sequencing .Conclusion The assay of HRM is simple ,rapid ,cost-effective ,and re-liable for the detection of ALDH2 and ADH1B polymorphism and it is worthy to be popularized .
Full text:
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Index:
WPRIM
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
International Journal of Laboratory Medicine
Year:
2018
Type:
Article