Function of RNA binding protein KSRP in type M5 acute myeloid leukemia cells / 基础医学与临床

ABSTRACT

Objective To investigate the expression of RNA binding protein KSRP in multiple acute myeloid leuke-mia(AML) patients. Methods KSRP expression among AML patients and normal controls was analyzed in gene expression omnibus (GEO) datasets and the cancer genome atlas(TCGA) database. KSRP expression was inhibi-ted by lentivirus-mediated gene transduction in THP-1 cells,cell proliferation and apoptosis were analyzed. Results KSRP has lower expression in t(15;17) acute promyelocytic leukemia while has higher expression in mixed lineage leukemia(MLL) translocated acute monocytic leukemia or acute myelomonocytic leukemia. KSRP knock-down sig-nificantly promoted apoptosis (P<0.01),as well as suppressed cell proliferation (P<0.01). In addition, overex-pression of miR-129 increased cell proliferation(P<0.05). Conclusions KSRP may regulate AML-M5 cell growth through promoting miR-129 processing.