Expression of gC1qR gene in ovarian cancer and its apoptotic mechanism / 临床与实验病理学杂志

ABSTRACT

Purpose To investigate the expression of globular C1q receptor (gC1qR) in ovarian cancer and to explore its potential molecular mechanism.Methods Retrospective analysis was made on 48 ovarian cancer tissues and ovarian cancer cell line SKOV3.Real time PCR and Western blot analysis were applied to detect the levels of gC1qR mRNA and gC1qR protein expression.The abilities of SKOV3 cells proliferation activity and quantity,migration and apoptosis were respectively assessed by M'TT,transwell assay and flow cytometry.Besides,the intracellular ROS was estimated via the fluorescence of H2DCFDA,the mitochondrial membrane potential was tested using a JC-1 probe,and the intracellular Ca2+ was assessed via Fluo-3/AM.Results The expressions of gC1qR gene was obviously decreased in the group of ovarian cancer tissues when compared with normal ovarian tissues group (2.61 ±0.34 vs 7.32 ± 1.25,0.20 ± 0.02 vs 0.67-± 0.06,P ＜ 0.001).Overexpression of gC1qR gene could result in significant up-regulation of ovarian cancer cell apoptosis and down-regulation in proliferation and migration,and showed significant cell apoptosis morphology.Simultaneously,the intracellular ROS and Ca2+ were obviously increased,and the mitochondrial membrane potential was obviously decreased.Conclusion gC1qR gene may play an important role in the apoptosis of ovarian cancer cells.In this process,gC1qR gene can induce apoptosis of ovarian cancer cells by inducing mitochondrial dysfunction.