Antibody Response of Mouse Splenocytes Using Mixture of Supernatants of Thymocytes, Adherent and Non-adherent Splenocytes: In-vitro Immunization-II
Journal of Korean Medical Science
; : 25-31, 1990.
Article
in En
| WPRIM
| ID: wpr-69573
Responsible library:
WPRO
ABSTRACT
We adapted one of the in-vitro immunization methods to induce antibody responses and confirmed the success of the immunization by enzyme-linked immunosorbent assay (ELISA) without hybridization. We have previously reported several methods of in-vitro immunization using different conditioned media. Here we introduce another method of in-vitro immunization using a mixture of three types of supernatant (thymocytes, and adherent and non-adherent splenocytes of mouse). Splenocytes were immunized in-vitro by a recombinant human growth hormone (rhGH) with the above conditioned media, and the results by ELISA showed a much higher optical density than the other in-vitro immunization methods that we had previously reported. Humoral responses as a result of in-vitro immunization to soluble antigens were easily confirmed by ELISA using the above-conditioned media. This finding indicates that any other conditions thought to be critical by other researches may not be essential for in-vitro immunization.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Spleen
/
Recombinant Proteins
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Lymphocyte Activation
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T-Lymphocytes
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Cell Adhesion
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Immunization
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Growth Substances
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Mice, Inbred BALB C
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Antibody Formation
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Antigens
Limits:
Animals
Language:
En
Journal:
Journal of Korean Medical Science
Year:
1990
Type:
Article