Your browser doesn't support javascript.
loading
Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment
J. vet. sci ; J. vet. sci;: 27-33, 2014.
Article in En | WPRIM | ID: wpr-69673
Responsible library: WPRO
ABSTRACT
In this study, we determined how rosiglitazone (RSG) differentially affected hippocampal neurogenesis in mice fed a low-fat diet (LFD) or high-fat diet (HFD; 60% fat). LFD and HFD were given to the mice for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle or RSG was administered orally once a day to both groups of mice. We measured cell proliferation and neuroblast differentiation in the subgranular zone of the dentate gyrus using Ki67 and doublecortin (DCX), respectively, as markers. In addition, we monitored the effects of RSG on the levels of DCX and brain-derived neurotrophic factor (BDNF) in hippocampal homogenates. At 8 weeks after the LFD feeding, the numbers of Ki67- and DCX-positive cells as well as hippocampal levels of DCX and BDNF were significantly decreased in the RSG-treated group compared to the vehicle-treated animals. In contrast, the numbers of Ki67- and DCX-positive cells along with hippocampal levels of DCX and BDNF in the HFD fed mice were significantly increased in the RSG-treated mice compared to the vehicle-treated group. Our data demonstrate that RSG can modulate the levels of BDNF, which could play a pivotal role in cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus.
Subject(s)
Key words
Full text: 1 Index: WPRIM Main subject: Neuropeptides / Immunohistochemistry / Cell Differentiation / Blotting, Western / Diet, Fat-Restricted / Dentate Gyrus / Brain-Derived Neurotrophic Factor / Ki-67 Antigen / Thiazolidinediones / Cell Proliferation Limits: Animals Language: En Journal: J. vet. sci Year: 2014 Type: Article
Full text: 1 Index: WPRIM Main subject: Neuropeptides / Immunohistochemistry / Cell Differentiation / Blotting, Western / Diet, Fat-Restricted / Dentate Gyrus / Brain-Derived Neurotrophic Factor / Ki-67 Antigen / Thiazolidinediones / Cell Proliferation Limits: Animals Language: En Journal: J. vet. sci Year: 2014 Type: Article