Protective effect of hyperbaric oxygen preconditioning on rat syngeneic kidney transplantation / 实用医学杂志

ABSTRACT

Objective To investigate the effect of hyperbaric oxygen preconditioning on syngeneic kidney transplantation in rats and to explore the potential mechanism. Methods Forty male SD rats were randomly divid-ed into control group(Sham group),kidney transplantation group(KTx group)and hyperbaric oxygen precondi-tioning group(HBO+KTx group). Kidney transplantation was performed 24 h after hyperbaric preconditioning. Rats were sacrificed 24 h after the transplantation.Serum creatinine(Scr)and blood urea nitrogen(BUN)were de-tected.The level of superoxide dismutase(SOD),malondialdehyde(MDA)and heme oxygenase-1(HO-1)in the graft was examined. Kidney pathological examination was performed after renal tissue fixed. Results Compared with the KTx group,HBO + KTx group showed lower Scr and BUN level. The MDA level was decreased and the SOD activity was increased significantly after hyperbaric oxygen preconditioning in HBO+KTx group.Renal tubu-lar necrosis was significantly reduced in HBO+KTx group.HO-1 level in HBO+KTx group was significantly high-er than that of KTx group. Conclusion Hyperbaric oxygen preconditioning can reduce lipid peroxidation,en-hance antioxidant enzyme activity and improve the level of HO-1 in graft tissue,thus attenuating kidney injury.