Determination of 10-Hydroxyl Carbamazepine in Human Serum by High Performance Liquid Chromatography / 医药导报

ABSTRACT

Objective To establish a method for the determination of 10-hydroxyl carbamazepine (MHD),which is an activity metabolite of oxcarbazepine in human serum. Methods Serum samples were detected by high performance liquid chromatography (HPLC) after being processed by methanol protein deposition.The chromatographic column was Agilent TC-C18 (4.6 mm × 250 mm, 5 μm), with the mobile phase of acetonitrile-10 mmol ? L-1 KH2 PO4 ( 33 : 67) at a flow rate of 1.0 mL?min-1 .The detection wavelength was 230 nm,and phenacetin was used as an internal standard. Results The average recovery range of low,middle and high (1.0,10.0,60.0 μg?mL-1 ) concentrations for MHD was from 100.3％ to 106.0％.The RSD of intra-day and inter-day was ≤5.8％ (n= 5) and ≤7.4％ (n= 5),respectively.The limit detection of analysis method was 0.1 μg?mL-1 .Regression equation was Y = 0.1308X+ 0.0679 ( r = 0.9966,n = 5). Serum samples remained stable at room temperature,freezing and freeze thawing condition. Conclusion This method is sensitive,accurate,simple and quick,and can be used for monitoring the oxcarbazepine metabolites MHD in serum for clinical and pharmacokinetic study.