Expression and significance of CD64 on monocyte subsets from patients with rheumatoid arthritis / 中国免疫学杂志

ABSTRACT

Objective:To investigate the expression of CD64 on monocyte subset from patients with rheumatoid arthritis (RA) and its significance and to clarify its role in the development of RA.Methods:The peripheral blood from 44 patients and 22 healthy controls (HC)were collected,the proportions of each monocyte subset and the CD64 expression on monocyte subsets were detected by flow cytometry.The proportions of each monocyte subset and the expression of TIGIT on monocyte subsets were compared between RA patients and HC.The correlations of CD64 expression on monocyte subsets with laboratory index were analyzed.The data were statistically analyzed.Results:(1)The proportion of intermediate monocytes in patients with RA was significantly higher than that in healthy volunteers (P＜0.01),while the proportion of classical and nonclassical monocytes in patients with RA was significantly lower than that in healthy volunteers (P＜0.05).(2) The expression of CD64 (MFI) on monocyte subsets were significantly elevated in RA patients compared to healthy volunteers (P＜0.05).(3)The expression of CD64 on classical monocytes and intermediate monocytes were positively correlated with DAS28 score (rs =0.308,P =0.044;rs =0.302,P =0.049).(4) The expression of CD64 (MFI) on monocyte subsets were positively correlated with ESR and CRP (rs =0.410,P=0.008;rs =0.475,P=0.003;rs =0.448,P=0.003;rs =0.473,P =0.004;rs =0.348,P =0.026;rs =0.340,P =0.042).(5) The expression of CD64 on classical monocytes and intermediate monocytes were significantly increased in patients with positive RF and ACPA respectively (P＜0.05).(6)The RA patients with high levels of CD64 on intermediate monocytes exhibited significantly higher levels of IL-6 compared with the RA patients with low levels of D64 on intermediate monocytes (P＜0.05).Conclusion:In RA,the expression of CD64.on monocyte subsets are elevated,and the expression of CD64 on classical monocytes and intermediate monocytes associated with the inflammatory markers,the production of antibodies and the disease activity.In addition,the expression of CD64 on intermediate monocytes associated with cytokine.