Simultaneous determination of nine constituents in Sanhuang Tablets by HPLC / 中成药

ABSTRACT

AIM To establish an HPLC method for the simultaneous content determination of berberine hydrochloride,baicalin,baicalein,wogonin,aloe emodin,rhein,emodin,chrysophanol,physcion in Sanhuang Tablets (Rhei Radix et Rhizoma,Scutellariae Radix,berberine hydrochloride).METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic Waters sunfire C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of methanol-acetonitrile-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 254 nm.RESULTS Nine constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 99.03％-100.03％ with the RSDs of 0.63％-1.07％.The contents of various constituents in ten batches of samples demonstrated obvious differences.CONCLUSION We should pay attention to the unstable quality of Sanhuang Tablets.