Hypothermia Inhibits Endothelium-Independent Vascular Contractility via Rho-kinase Inhibition
Biomolecules & Therapeutics
; : 139-145, 2018.
Article
in En
| WPRIM
| ID: wpr-713582
Responsible library:
WPRO
ABSTRACT
The present study was undertaken to investigate the influence of hypothermia on endothelium-independent vascular smooth muscle contractility and to determine the mechanism underlying the relaxation. Denuded aortic rings from male rats were used and isometric contractions were recorded and combined with molecular experiments. Hypothermia significantly inhibited fluoride-, thromboxane A2-, phenylephrine-, and phorbol ester-induced vascular contractions regardless of endothelial nitric oxide synthesis, suggesting that another pathway had a direct effect on vascular smooth muscle. Hypothermia significantly inhibited the fluoride-induced increase in pMYPT1 level and phorbol ester-induced increase in pERK1/2 level, suggesting inhibition of Rho-kinase and MEK activity and subsequent phosphorylation of MYPT1 and ERK1/2. These results suggest that the relaxing effect of moderate hypothermia on agonist-induced vascular contraction regardless of endothelial function involves inhibition of Rho-kinase and MEK activities.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phosphorylation
/
Relaxation
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Rho-Associated Kinases
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Fluorides
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Hypothermia
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Isometric Contraction
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Muscle, Smooth, Vascular
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Nitric Oxide
Limits:
Animals
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Humans
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Male
Language:
En
Journal:
Biomolecules & Therapeutics
Year:
2018
Type:
Article