Phenotypic and Genomic Characterization of AmpC-Producing Klebsiella pneumoniae From Korea
Annals of Laboratory Medicine
; : 367-370, 2018.
Article
in En
| WPRIM
| ID: wpr-715636
Responsible library:
WPRO
ABSTRACT
The prevalence of multidrug-resistant gram-negative bacteria has continuously increased over the past few years; bacterial strains producing AmpC β-lactamases and/or extended-spectrum β-lactamases (ESBLs) are of particular concern. We combined high-resolution whole genome sequencing and phenotypic data to elucidate the mechanisms of resistance to cephamycin and β-lactamase in Korean Klebsiella pneumoniae strains, in which no AmpC-encoding genes were detected by PCR. We identified several genes that alone or in combination can potentially explain the resistance phenotype. We showed that different mechanisms could explain the resistance phenotype, emphasizing the limitations of the PCR and the importance of distinguishing closely-related gene variants.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phenotype
/
Drug Resistance, Microbial
/
Polymerase Chain Reaction
/
Prevalence
/
Genome
/
Gram-Negative Bacteria
/
Klebsiella
/
Klebsiella pneumoniae
/
Korea
Type of study:
Prevalence_studies
Country/Region as subject:
Asia
Language:
En
Journal:
Annals of Laboratory Medicine
Year:
2018
Type:
Article