Cellular Response of Anodized Titanium Surface by Poly(Lactide-co-Glycolide)/Bone Morphogenic Protein-2
Tissue Engineering and Regenerative Medicine
; (6): 591-599, 2018.
Article
in En
| WPRIM
| ID: wpr-717543
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
The purpose of this study is to examine physical characteristics of and initial biological properties to anodized titanium treated with poly(D,L-lactide-co-glycolide) (PLG) mixed with recombinant human bone morphogenic protein-2 (rhBMP-2).METHODS:
Titanium specimens were prepared in groups of four as follows group NC was anodized under 300 V as control; group PC was anodized then dropped and dried with solution 0.02 ml PLG; group D was anodized then dropped and dried with solution 0.02 ml PLG/rhBMP-2 (3.75 µg per disc); and group E was anodized then coated with 0.02 ml PLG/rhBMP-2 (3.75 µg per disc) by electrospray. Human osteoblastic-like sarcoma cells were cultured. Cell proliferation and alkaline phosphatase (ALP) activity test were carried out. Runx-2 gene was investigated by the reverse transcription-polymerase chain reaction. Immunofluorescence outcome of osteogenic proteins was observed.RESULTS:
After 3 days, there were significantly higher proliferations compared rhBMP-2 loaded titanium discs with rhBMP-2 unloaded discs. The ALPase activity on rhBMP-2 loaded titanium discs was significantly higher than in rhBMP-2 unloaded discs. The expression level of Runx2 mRNA presented the highest on the PLG/rhBMP-2-coated surface.CONCLUSION:
PLG polymers mixed with rhBMP-2 might improve proliferation, differentiation and osteogenic protein formation of cells on the anodized titanium.Key words
Full text:
1
Index:
WPRIM
Main subject:
Polymers
/
Sarcoma
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Titanium
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RNA, Messenger
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Cell Differentiation
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Fluorescent Antibody Technique
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Cell Proliferation
/
Alkaline Phosphatase
Limits:
Humans
Language:
En
Journal:
Tissue Engineering and Regenerative Medicine
Year:
2018
Type:
Article