Induction of ER Stress-Mediated Apoptosis by alpha-Lipoic Acid in A549 Cell Lines
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 1-10, 2012.
Article
in English
| WPRIM
| ID: wpr-71955
ABSTRACT
BACKGROUND:
alpha-Lipoic acid (alpha-LA) has been studied as an anticancer agent as well as a therapeutic agent for diabetes and obesity. We performed this study to evaluate the anticancer effects and mechanisms of alpha-LA in a lung cancer cell line, A549. MATERIALS ANDMETHODS:
alpha-LA-induced apoptosis of A549 cells was detected by fluorescence-activated cell sorting analysis and a DNA fragmentation assay. Expression of apoptosis-related genes was analyzed by western blot and reverse transcription-polymerase chain reaction analyses.RESULTS:
alpha-LA induced apoptosis and DNA fragmentation in A549 cells in a dose- and time-dependent manner. alpha-LA increased caspase activity and the degradation of poly (ADP-ribose) polymerase. It induced expression of endoplasmic reticulum (ER) stress-related genes, such as glucose-regulated protein 78, C/EBP-homologous protein, and the short form of X-box binding protein-1, and decreased expression of the anti-apoptotic protein, X-linked inhibitor of apoptosis protein. Reactive oxygen species (ROS) production was induced by alpha-LA, and the antioxidant N-acetyl-L-cysteine decreased the alpha-LA-induced increase in expression of apoptosis and ER stress-related proteins.CONCLUSION:
alpha-LA induced ER stress-mediated apoptosis in A549 cells via ROS. alpha-LA may therefore be clinically useful for treating lung cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Acetylcysteine
/
Cell Line
/
Blotting, Western
/
Thioctic Acid
/
Cell Death
/
Reactive Oxygen Species
/
Apoptosis
/
Endoplasmic Reticulum
/
X-Linked Inhibitor of Apoptosis Protein
/
DNA Fragmentation
Language:
English
Journal:
The Korean Journal of Thoracic and Cardiovascular Surgery
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS