Immunomodulation Effect of the Allogeneic Cellular Components after Transfusion
Korean Journal of Hematology
; : 375-381, 2007.
Article
in Ko
| WPRIM
| ID: wpr-720991
Responsible library:
WPRO
ABSTRACT
BACKGROUND: Blood transfusion is important for life saving treatment in many patients with tolerable adverse effects. Some data suggest that transfusions might cause an increased risk for post-operative infections and a higher relapse or mortality rate in cancer patients. We investigated whether immune dysfunction might result after transfusions from the cellular components. METHODS: We studied 5-week-old mice BALB/c (H-2d, donor), C3H/He (H-2k, recipient), and C57/BL (H-2b, third party). We obtained irradiated spleen cells (SP) from the BALB/c or C57/BL, and injected them into the C3H/He with intraperitoneal IL-2 administration. After 24 hours, we obtained bone marrow (BM), thymus and SP. We identified mixed lymphocyte proliferation (MLR) by the BrdU method and we used irradiated BALB/c SP, as a stimulator for that trial. For the analysis of immune cells, we analyzed the cell surface markers from each organ. For cytokines, we identified TNF-alpha, IFN-gamma, TGF-beta, and IL-10 by ELISA from the supernatant of the MLR. RESULTS: The cell proliferation decreased according to specific H-2 complexes. There were increased CD4+CD25+ cells in the thymus. For the paracrine effects, the B-C3H SP showed ratio-dependent inhibitory effects, although the C-C3H SP inhibited some cell proliferation. There was no difference in the IFN-gamma, TNF-alpha and TGF-beta between the control and experimental groups. However, IL-10 was higher in the 1:10 ratio mixture in the control and transfused SP compared to the other groups. CONCLUSION: The results of this study suggested that the cellular components in transfusions might contribute to the immune regulatory effects by CD4+CD25+ cells after 24 hours.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Recurrence
/
Spleen
/
Thymus Gland
/
Blood Transfusion
/
Bone Marrow
/
Bromodeoxyuridine
/
Enzyme-Linked Immunosorbent Assay
/
Lymphocytes
/
Mortality
/
Cytokines
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
Ko
Journal:
Korean Journal of Hematology
Year:
2007
Type:
Article