Toll-like Receptor3-mediated Induction of Chemokines in Salivary Epithelial Cells
The Korean Journal of Physiology and Pharmacology
; : 235-240, 2010.
Article
in En
| WPRIM
| ID: wpr-727794
Responsible library:
WPRO
ABSTRACT
Toll-like receptors (TLRs) functionally expressed in salivary epithelial cells, but their roles remain elusive. Among TLRs family, TLR3 is activated by dsRNA, a byproduct of viral infection. The aim of this study was to investigate the role of TLR3 in the inflammatory immune responses using HSG cells. Reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR and ELISA were performed to identify expression of TLRs and TLR3-mediated chemokine inductions. The chemotaxis assay of activated T lymphocytes was also performed. Treatment of HSG cells with polyinosinic: polycytidylic acid (poly(I:C)) significantly increased interferon-gamma-inducible protein 10 (IP-10), interferoninducible T-cell alpha chemoattractant (I-TAC), and regulated on activation, normal T-cells expressed and secreted (RANTES) gene expressions in a concentration-dependent manner. Anti-TLR3 antibody blocked the increases of IP-10 and I-TAC genes. Poly(I:C)-induced increases of IP-10 and I-TAC were also confirmed at protein levels from cell lysates, but their release into extracellular medium was detected only in IP-10. We found that the culture media from HSG cells stimulated with poly(I:C) significantly increases T lymphocyte migration. Our results suggest that TLR3 plays an important role in chemokine induction, particularly IP-10, in salivary epithelial cells.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Enzyme-Linked Immunosorbent Assay
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Lymphocytes
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T-Lymphocytes
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Gene Expression
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Chemotaxis
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Chemokines
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Culture Media
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Epithelial Cells
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Toll-Like Receptors
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Real-Time Polymerase Chain Reaction
Limits:
Humans
Language:
En
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2010
Type:
Article