p38 MAPK and NF-kappaB are required for LPS-induced RANTES production in immortalized murine microglia (BV-2)
The Korean Journal of Physiology and Pharmacology
; : 339-346, 2000.
Article
in En
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| ID: wpr-728144
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ABSTRACT
Using murine immortalized microglial cells (BV-2), we examined the regulation of RANTES production stimulated by lipopolysaccharide (LPS), focusing on the role of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-kappaB. The result showed that RANTES (regulated upon activation of normal T cell expressed and secreted) was induced at the mRNA and protein levels in a dose- and time-dependent manner in response to LPS. From investigations of second messenger pathways involved in regulating the secretion of RANTES, we found that LPS induced phosphorylation of extracellular signal-regulated kinase (Erk), p38 MAPK and c-Jun-N-terminal kinase (JNK), and activated NF-kappaB. To determine whether this MAPK phosphorylation is involved in LPS-stimulated RANTES production, we used specific inhibitors for p38 MAPK and Erk, SB 203580 and PD 98059, respectively. LPS-induced RANTES production was reduced approximately 80% at 25 micrometer of SB 203580 treatment. But PD 98059 did not affect RANTES production. Pyrrolidine-dithiocarbamate (PDTC), NF-kappaB inhibitor, reduced RANTES secretion. These results suggest that LPS-induced RANTES production in microglial cells (BV-2) is mainly mediated by the coordination of p38 MAPK and NF-kappaB cascade.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phosphorylation
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Phosphotransferases
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Protein Kinases
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RNA, Messenger
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Second Messenger Systems
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NF-kappa B
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Microglia
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Chemokine CCL5
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P38 Mitogen-Activated Protein Kinases
Language:
En
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2000
Type:
Article