Roles of ERK and NF-kappa B in Interleukin-8 Expression in Response to Heat Shock Protein 22 in Vascular Smooth Muscle Cells
The Korean Journal of Physiology and Pharmacology
; : 171-176, 2008.
Article
in En
| WPRIM
| ID: wpr-728391
Responsible library:
WPRO
ABSTRACT
Heat shock proteins (HSPs) serve as molecular chaperones and play a role in cell protection from damage in response to stress stimuli. The aim of this article is to investigate whether HSP22 affects IL-8 expression in vascular smooth muscle cells (VSMCs), and which cellular factors are involved in the HSP-mediated IL-8 induction in that cell type in terms of mitogen activated protein kinase (MAPK) and transcription element. Exposure of aortic smooth muscle cells (AoSMCs) to HSP22 not only enhanced IL-8 release but also induced IL-8 transcript via promoter activation. HSP22 activated ERK and p38 MAPK in AoSMCs. HSP22-induced IL-8 release was inhibited by U0126, but not by SB202190. A mutation in the IL-8 promoter region at the binding site of NF-kappa B, but not AP-1 or C/EBP, impaired promoter activation in response to HSP22. Delivery of I kappa B, but not dominant negative c-Jun, lowered HSP22-induced IL-8 release from AoSMCs. These results suggest that HSP22 induces IL-8 in VSMCs via ERK1/2, and that transcription factor NF-kB may be required for the HSP22-induced IL-8 up-regulation.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Protein Kinases
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Pyridines
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Shock
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Binding Sites
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Butadienes
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Proteins
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Interleukin-8
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NF-kappa B
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Promoter Regions, Genetic
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Transcription Factor AP-1
Language:
En
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2008
Type:
Article