Mediation of intracellular Ca2+ in the phospholipase A2-induced cell proliferation in human neuroblastoma cells
The Korean Journal of Physiology and Pharmacology
; : 411-417, 1998.
Article
in En
| WPRIM
| ID: wpr-728699
Responsible library:
WPRO
ABSTRACT
The role of phospholipase A2 (PLA2) in tumor cell growth was investigated using SK-N-MC human neuroblastoma cells. 4-Bromophenacyl bromide (BPB) and mepacrine (Mep), known PLA2 inhibitors, suppressed growth of the tumor cells in a dose-dependent manner without a significant cytotoxicity. Melittin (Mel), a PLA2 activator, enhanced the cell growth in a concentration-dependent fashion. The growth-enhancing effects of Mel were significantly reversed by the co-treatment with PLA2 inhibitors. In addition, Mel induced intracellular Ca2+ release from internal stores like as did serum, a known intracellular Ca2+ agonist in the tumor cells. Intracellular Ca2+ release induced by these agonists was significantly blocked by PLA2 inhibitors at growth-inhibitory concentrations. Arachidonic acid (AA), a product of the PLA2-catalyzed reaction, induced cell growth enhancement and intracellular Ca2+ release. These effects of AA were significantly blocked by BAPTA/AM, an intracellular Ca2+ chelator. Taken together, these results suggest that the modulation of PLA2 activity may be one of the regulatory mechanisms of cell growth in human neuroblastoma cells. Intracellular Ca2+ may act as a key mediator in the PLA2-induced growth regulation.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phospholipases
/
Quinacrine
/
Negotiating
/
Arachidonic Acid
/
Cell Proliferation
/
Phospholipases A2
/
Melitten
/
Neuroblastoma
Limits:
Humans
Language:
En
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
1998
Type:
Article