Genomic Organization of ancop Gene for alpha-COP Homolog from Aspergillus nidulans
Mycobiology
; : 171-176, 2000.
Article
in En
| WPRIM
| ID: wpr-729318
Responsible library:
WPRO
ABSTRACT
We have cloned a alpha-COP homolog, ancop, from Aspergillus nidulans by colony hybridization of chromosome specific library using alpha-COP homologous fragment as a probe. The probe DNA was amplified with degenerated primers designed by comparison of conserved region of the amino acid sequences of Saccharomyces cerevisiae alpha-COP, Homo sapiens HEP-COP, and Drosophila melanogaster alpha-COP. Full length cDNA clone was also amplified by RT-PCR. Comparison of genomic DNA sequence with cDNA sequence obtained by RT-PCR revealed 7 introns. Amino acid sequence similarity search of the anCop with other alpha-COPs gave an overall identity of 52% with S. cerevisiae, 47% with human and bovine, 45% with Drosophila and Arabidopsis . In upstream region from the transcription start site, a putative TATA and CAAT motif were also identified.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Aspergillus
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Aspergillus nidulans
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Saccharomyces cerevisiae
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DNA
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Base Sequence
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Introns
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Amino Acid Sequence
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Clone Cells
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Sequence Homology
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Arabidopsis
Limits:
Humans
Language:
En
Journal:
Mycobiology
Year:
2000
Type:
Article