Analysis of the Chemical Constituents of Agaricus brasiliensis
Mycobiology
; : 50-54, 2008.
Article
in En
| WPRIM
| ID: wpr-729560
Responsible library:
WPRO
ABSTRACT
This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty acids by GC were analyzed. The immuno-stimulating substance from A. blasiliensis was extracted with hot water and purified by ethanol precipitation. It underwent ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. Through GP-HPLC, the substance was found to be homogeneous. Its chemical structure was determined by 13C-NMR. Fatty acids, organic acids, and sugar alcohol composition consisted exclusively of linoleic acid, fumaric acid and mannitol, respectively. The amino acids were mainly glutamic acid, glycine, and arginine. By 13C-NMR analysis, the immuno-stimulating substance was identified as beta-(1-->3) (1-->6)-glucan, composed of a backbone with (1-->3)-linked D-glucopyranosyl residues branching a (1-->6)-linked D-glucopyranosyl residue. The beta-glucan from A. blasiliensis showed pronounced immuno-stimulating activity on the antibody-production ability of B-lymphocytes by the hemolytic suspension assay. In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Arginine
/
Agaricus
/
Carbohydrates
/
Water
/
B-Lymphocytes
/
Chromatography, Gel
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Chromatography, High Pressure Liquid
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Chromatography, Ion Exchange
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Glutamic Acid
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Linoleic Acid
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Mycobiology
Year:
2008
Type:
Article