Changes in expression of gamma-aminobutyric acid receptor subunit genes in dorsal root ganglia in a mouse model of neuropathic pain / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the changes in the expression of gamma-aminobutyric acid (GABA) receptor subunit genes in the dorsal root ganglia (DRG) in a mouse model of neuropathic pain. Methods Experiment Ⅰ Twenty-four male C57BL6 mice, aged 8 weeks, weighing 25-30 g, were di-vided into sham operation group (group Sham, n=12) and neuropathic pain group (group NP, n=12) by using a random number table method. Neuropathic pain was produced by bilateral L4 spinal nerve ligation in anesthetized mice in group NP . The mechanical pain threshold of bilateral hindpaws was measured at 1 day before establishing the model and 7 days after establishing the model. Mice were then sacrificed and DRGs of the bilateral L4 were removed to perform transcriptome sequencing and to analyze the expression of GABA receptor subunit genes. Experiment Ⅱ Twenty-four male C57BL6 mice, aged 8 weeks, weighing 25-30 g, were studied. Neuropathic pain was produced by the left L4 spinal nerve ligation in anesthetized mice. Six mice were selected at 1 day before establishing the model and 3, 7 and 14 days after establishing the model, and the mechanical pain threshold of the left hindpaw was measured. Mice were then sacrificed and DRGs of the left L4 were removed to verify the expression of differentially expressed GABA receptor subunitgenes described in experiment Ⅰby quantitative real-time polymerase chain reaction. Results ExperimentⅠ Compared with group Sham, the mechanical pain threshold was significantly increased, the expression of Gabra1, Gabra2, Gabrb3, Gabrg2, Gabbr1 and Gabbr2 in DRGs was down-regulated, and the expres-sion of Gabrg1 in DRGs was up-regulated at 7 days after establishing the model in group NP ( P<0. 05 or 0. 01) . Experiment Ⅱ Compared with the baseline at 1 day before establishing the model, the mechanical pain threshold was significantly increased, and the expression of Gabra1, Gabra2, Gabrb3, Gabrg2, Gab-br1 and Gabbr2 in DRGs was down-regulated at each time point after establishing the model ( P<0. 05 or 0. 01) , and no significant change was found in the expression of Gabrg1 in DRGs at each time point after establishing the model ( P>0. 05) . Conclusion The expression of GABA receptor subunit genes Gabra1, Gabra2, Gabrb3, Gabrg2, Gabbr1 and Gabbr2 in DRGs is down-regulated, and the expression of Gabrg1 in DRGs is up-regulated in a mouse model of neuropathic pain.