Evaluation of two different enzyme-linked immunosorbent assay for severe fever with thrombocytopenia syndrome virus diagnosis
Clinical and Experimental Vaccine Research
;
: 82-86, 2018.
Article
in English
| WPRIM
| ID: wpr-739633
ABSTRACT
To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strains compared with those of Gn-based ELISA tests. However, Gn-based ELISA showed more genotype specificity and specificity. These result suggested that NP-based ELISA test could be applicable for general sero-prevalence studies of SFTSV infections, while Gn-based ELISA could be applicable for a certain specific genotype sero-prevalence study.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Thrombocytopenia
/
Enzyme-Linked Immunosorbent Assay
/
Sensitivity and Specificity
/
Nucleocapsid Proteins
/
Diagnosis
/
Fever
/
Genotype
Type of study:
Diagnostic study
Language:
English
Journal:
Clinical and Experimental Vaccine Research
Year:
2018
Type:
Article
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