Inhibitory effect of saikosaponin-d on the proliferation and migration of human breast cancer MDA-MB-231 cells / 国际药学研究杂志

ABSTRACT

Objective To investigate the effect and related mechanism of saikosaponin-d (SS-d) on the proliferation and migration of breast cancer MDA-MB-231 cells. Methods The MDA-MB-231 cells were treated with varying concentrations of SS-d.CCK-8 and colony formation analysis were used to evaluate the proliferation ability of MDA-MB-231 cells;3D cell culture, wound healing assay and Transwell migration and invasion assays were used to analyze the ability of cell migration and invasion;flow cytometry was utilized to analyze the cell cycle and apoptosis;semi-quantitative RT (qRT)-PCR and Western blot were used to explore the action mechanism of SS-d on the cell migration and invation. Results SS-d inhibited the proliferation and colony formation ability of MDA-MB-231 cells, both dose-and time-dependently;migration and invasion abilities of the MB-231 cells were also significantly inhibited by the treatment with SS-d (P＜0.01);the treatment with SS-d caused the significant cell cycle arrest at G1 phase and also significant apoptosis-induction in MDA-MB-231 cells;SS-d could up-regulate the expression of P21, P27 E-cadherin (P＜0.01), but down-regulate the expression of Bcl-2, vimentin and N-cadherin (P＜0.01), all detected in both the m RNA and protein levels. Conclusion SS-d was likely to be a potential antitumor drug for the breast cancer therapy to inhibit the proliferation and metastasis of the cancer cells.