Responses of prostate carcinoma cell line to fibroblast conditioned medium treated with serum and dihydrotestosterone / 대한비뇨기과학회지

ABSTRACT

To investigate the biological effect of fibroblast conditioned medium on the proliferation of epithelial cancer cell in vitro with serum and/or dihyrotestosterone(DHT), media conditioned with 3 normal fibroblast cell lines(NUGM, HPF and 3T3) and 1 transformed fibroblast(TUGM) were assayed on the growth of hormone-independent prostate epithelial cancer cell line, PC-3 and quantified by cell counting. The results demonstrated that the TUGM conditioned medium significantly enhanced the proliferation of PC-3 more than the other normal fibroblasts conditioned media in case of not contained serum. But in contrast, normal fibroblasts conditioned media. cultured with 0.4% charcoal stripped calf serum showed marked stimulation of PC-3 proliferation, and TUGM conditioned medium treated with 5% serum. Addition of DHT revealed significant growth enhancement only in case of conditioned medium obtained from NUGM and HPF without serum. These suggest the possibilities that the addition of serum can elicit the production of different growth factors, either stimulating or inhibiting in one cell type of fibroblast and the proliferation of androgen-independent prostate cancer cells in vitro can be regulated by androgenic stimulation via androgen-responsive fibroblast.