The role of intracellular Ca2+ release in olfactory signal transduction / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 940-944, 2010.
Article
in Zh
| WPRIM
| ID: wpr-747498
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To setup the real time monitor system of the concentration of free intracellular calcium ([Ca2+]i) of olfactory receptor neurons (ORNs) cultured from olfactory epithelium explant, and to analyze the role of several important components in olfactory signal transduction.@*METHOD@#The [Ca2+]i of the cultured ORNs was determined by fluorescence microscopy using the fluorescent calcium indicator, Fura-2 AM, and calculated by means of dual-wavelength ratiometric method. Forskolin and IBMX were used to stimulate the cultured ORNs respectively. The source of corresponding [Ca2+]i elevation was studied by the depletion of extracellular or intracellular calcium.@*RESULT@#The [Ca2+]i of silent ORNs was (58.5 +/- 12.8) nmol/L. Forskolin or IBMX stimulation led to reversible accumulation of [Ca2+]i in the ORNs. The [Ca2+]i change was abolished with the removal of extracellular Ca2+ and un-affected by treatment with thapsigargin.@*CONCLUSION@#A system to visualize and quantify [Ca2+]i of the ORNs was established. [Ca2+]i of the ORNs was regulated by second messenger gated calcium channels.
Full text:
1
Index:
WPRIM
Main subject:
Cells, Cultured
/
Calcium
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Olfactory Receptor Neurons
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Cyclic AMP
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Calcium Signaling
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Metabolism
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Mice, Inbred BALB C
Limits:
Animals
Language:
Zh
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2010
Type:
Article