Inhibition of growth and proliferation of Hep-2 cells by targeting c-myc gene using small interference RNA technology / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 399-402, 2008.
Article
in Zh
| WPRIM
| ID: wpr-749060
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of small interfering RNA (siRNA) targeting c-myc gene in Hep-2 cells.@*METHOD@#siRNA targeting c-myc mRNA was designed and synthesized. In vitro cultured Hep-2 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by MTT, morphology, real time PCR assay.@*RESULT@#1) The MTT result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication; 2) The real time PCR result showed c-myc at mRNA level inhibition ratio at 94% in group S3; 3) The morphology result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication, the cell heteromorphism was diminished.@*CONCLUSION@#siRNA targeting c-myc can remarkably suppress the Hep-2 cell growth and multiplication.
Full text:
1
Index:
WPRIM
Main subject:
Transfection
/
Cell Division
/
Genes, myc
/
Apoptosis
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Gene Targeting
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RNA, Small Interfering
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Cell Line, Tumor
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Cell Proliferation
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Genetics
Limits:
Humans
Language:
Zh
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2008
Type:
Article