High glucose induced glucose-key metabolic enzyme expression in lens epithelial cells / 国际眼科杂志(Guoji Yanke Zazhi)

ABSTRACT

@#AIM:To investigate the expression of glucose key metabolic enzymes in human lens epithelial cells(HLEB3)induced by high glucose.<p>METHODS:HLEB3 cells cultured <i>in vitro</i> were divided into normal control group(DMEM medium containing 5mmol/L of glucose), oxidative stress group(DMEM medium containing 5mmol/L of glucose and 200μmol/L of hydrogen peroxide), high glucose induction group(DMEM medium containing 30mmol/L of glucose). Apoptosis and cells were detected 24h after culture. Activity and expression of six key glucose metabolic enzymes(fructose-6-phosphate kinase-1, pyruvate kinase, hexokinase, citrate synthase, α-ketoglutarate dehydrogenase and 6-phosphate glucose dehydrogenase)were studied.<p>RESULTS:Apoptosis of HLEB3 cells was induced by high glucose. The cell viability of high glucose-induced group(63.43%±3.40%)was lower than that of normal control group(100.00%±0.00%)and oxidative stress group(91.90%±5.11%), and the expression levels of six key enzymes of glucose metabolism in high glucose-induced group were lower than that of normal control group and oxidative stress group(all <i>P</i><0.05).<p>CONCLUSION:High glucose can induce the expression of glucose-key metabolizing enzymes in HLEB3 cells to decrease, induce apoptosis and affect cell activity.