HID-1 is a peripheral membrane protein primarily associated with the medial- and trans- Golgi apparatus
Protein & Cell
; (12): 74-85, 2011.
Article
in En
| WPRIM
| ID: wpr-757120
Responsible library:
WPRO
ABSTRACT
Caenorhabditis elegans hid-1 gene was first identified in a screen for mutants with a high-temperature-induced dauer formation (Hid) phenotype. Despite the fact that the hid-1 gene encodes a novel protein (HID-1) which is highly conserved from Caenorhabditis elegans to mammals, the domain structure, subcellular localization, and exact function of HID-1 remain unknown. Previous studies and various bioinformatic softwares predicted that HID-1 contained many transmembrane domains but no known functional domain. In this study, we revealed that mammalian HID-1 localized to the medial- and trans- Golgi apparatus as well as the cytosol, and the localization was sensitive to brefeldin A treatment. Next, we demonstrated that HID-1 was a peripheral membrane protein and dynamically shuttled between the Golgi apparatus and the cytosol. Finally, we verified that a conserved N-terminal myristoylation site was required for HID-1 binding to the Golgi apparatus. We propose that HID-1 is probably involved in the intracellular trafficking within the Golgi region.
Full text:
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Index:
WPRIM
Main subject:
Pharmacology
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Brefeldin A
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Trans-Golgi Network
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Protein Transport
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Cytosol
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Vesicular Transport Proteins
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Cell Line, Tumor
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Intracellular Space
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Membrane Proteins
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Metabolism
Limits:
Animals
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Humans
Language:
En
Journal:
Protein & Cell
Year:
2011
Type:
Article