Histopathological and microbiological findings in buffalo chronic mastitis: evidence of tertiary lymphoid structures
Journal of Veterinary Science
; : e28-2019.
Article
in En
| WPRIM
| ID: wpr-758910
Responsible library:
WPRO
ABSTRACT
Buffalo mastitis is an important economic problem in southern Italy, causing qualitative/quantitative alterations in milk and resulting in economic losses due to the sub-clinical course and chronic evolution. We investigated 50 udders of slaughtered buffaloes and subjected them to effectual microbiological screening to evaluate macro and microscopic mammary gland changes, immune-characterize the cell infiltrates, and compare the degree of tissue inflammation with somatic cell counts. Numerous Gram-positive and Gram-negative bacteria were isolated from all samples, majority of which were environmental mastitis pathogens. Histological features referable to chronic mastitis were observed in 92% udders. Lymphocytes, plasma cells and macrophages were found to evolve into aggregates in 48% udders, which often organized to form tertiary lymphoid structures (TLSs). A predominance of interstitial CD8+ over CD4+ lymphocytes and, in TLSs, scattered CD8+ lymphocytes in the mantle cells and CD79+ lymphocytes in germinal centers, were evidenced. Environmental pathogens are known to persist and cause chronic inflammatory changes in buffaloes, where CD8+ lymphocytes play an important role by controlling the local immune response. Moreover, the TLSs evidenced here for the first time in buffalo mastitis, could play a role in maintaining immune responses against persistent antigens, thereby contributing in determining the chronic course of mastitis.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Plasma Cells
/
Buffaloes
/
Lymphocytes
/
Cell Count
/
Mass Screening
/
Germinal Center
/
Mammary Glands, Human
/
Milk
/
Gram-Negative Bacteria
/
Inflammation
Type of study:
Diagnostic_studies
/
Prognostic_studies
/
Qualitative_research
/
Screening_studies
Limits:
Animals
Country/Region as subject:
Europa
Language:
En
Journal:
Journal of Veterinary Science
Year:
2019
Type:
Article