Progress in gene knockout mice / 生物工程学报
Chinese Journal of Biotechnology
; (12): 784-794, 2019.
Article
in Zh
| WPRIM
| ID: wpr-771331
Responsible library:
WPRO
ABSTRACT
The establishment and development of gene knockout mice have provided powerful support for the study of gene function and the treatment of human diseases. Gene targeting and gene trap are two techniques for generating gene knockout mice from embryonic stem cells. Gene targeting replaces endogenous knockout gene by homologous recombination. There are two ways to knock out target genes promoter trap and polyA trap. In recent years, many new gene knockout techniques have been developed, including Cre/loxP system, CRISP/Cas9 system, latest ZFN technology and TALEN technology. This article focuses on the several new knockout mouse techniques.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Mice, Knockout
/
Gene Targeting
/
Disease Models, Animal
/
Embryonic Stem Cells
/
Gene Knockout Techniques
/
Homologous Recombination
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2019
Type:
Article