Effects of large tumor suppressor homolog 2 gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 609-612, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-772450
ABSTRACT
OBJECTIVE@#To investigate the effect of large tumor suppressor homolog 2 (LATS2) gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma (OSCC).@*METHODS@#Lentivirous particles were transferred into SCC-25 cell to upregulate LATS2 gene expression. Cell proliferation was detected by CCK-8 assay. Apoptosis was detected through flow cytometry. The expression changes of Bax, Bcl-2, and LATS2 were analyzed by Western blot.@*RESULTS@#Gene transfection increased LATS2 expression. Compared with the control group and pEGFP-control group, SCC-25 cell proliferation in the pGFP-LATS2 group was inhibited, whereas the apoptosis ratio increased (P<0.05). Bcl-2 expression decreased, and Bax expression increased.@*CONCLUSIONS@#Overexpression of LATS2 could inhibit SCC-25 cell proliferation and induce apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Mouth Neoplasms
/
Carcinoma, Squamous Cell
/
Gene Expression Regulation, Neoplastic
/
Protein Serine-Threonine Kinases
/
Apoptosis
/
Tumor Suppressor Proteins
/
Cell Line, Tumor
/
Cell Proliferation
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2018
Type:
Article
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