Saliva of periodontitis patients promotes macrophage differentiation and activation / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 508-513, 2018.
Article
in Zh
| WPRIM
| ID: wpr-772467
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#The aim of this study was to investigate the effect of saliva of patients with chronic periodontitis (CPD) on the differentiation, activation, and secretion of osteoclast-maturing mediators of macrophages.@*METHODS@#A total of 40 saliva samples were collected from healthy donors (n=20) and severe periodontitis patients (n=20). Peripheral blood mononuclear cells (PBMCs) and THP-1 monocyte line cells were challenged with 15% saliva for 5 days. The phenotype, surface marker, and phagocytosis of macrophages were analyzed by flow cytometry and microscopy. Osteoclast-maturing mediators were assayed by using enzyme-linked immunosorbent assay (ELISA) kits.@*RESULTS@#When PBMCs were treated with CPD saliva for 5 days, 61.25%±11.33% of cells were transformed into large granular cells; 86.78%±13.69% of large granular cells were identified as CD14⁺⁺CD16⁺ macrophages. When THP-1 cells were treated with CPD saliva, most cells attached to the bottom of cell culture plates, thereby exhibiting macrophage morphology and releasing additional osteoclast-maturing mediators. Furthermore, the phagocytosis of THP-1 cells considerably increased in the presence of CPD saliva (66.35%±9.67%) compared with medium control (33.33%±7.52%), or healthy saliva (40.71%±3.52%).@*CONCLUSIONS@#Saliva from patients with CPD can induce macrophage differentiation, activate phagocytose microorganisms, and secrete osteoclast-maturing mediators.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Periodontitis
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Saliva
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Leukocytes, Mononuclear
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Monocytes
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Cell Differentiation
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Allergy and Immunology
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Macrophages
Limits:
Humans
Language:
Zh
Journal:
West China Journal of Stomatology
Year:
2018
Type:
Article