Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674 / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 427-437, 2019.
Article
in English
| WPRIM
| ID: wpr-773387
ABSTRACT
OBJECTIVE@#This study aimed to characterize the diagnostic and vaccine potential of a novel Mycobacterium tuberculosis antigen Rv0674.@*METHODS@#To evaluate the diagnostic potential and antigenicity of Rv0674, IgG was evaluated using ELISA and interferon (IFN)-γ was done by using ELISpot assay among TB patients and healthy donors. For immunogenicity evaluation, BALB/c mice were immunized with Rv0674. Cytokine production was determined by cytokine release assay using an ELISA kit, and the antibodies were tested using ELISA.@*RESULTS@#The results of serum Elisa tests showed that Rv0674 specific immunoglobulin G (IgG) response was higher in TB patients than negative controls. And Rv0674 had good performance in serological test with sensitivity and specificity of 77.1% and 81.1%, respectively. While it shows poor sensitivity and specificity of 26.23% and 79.69% for IFN-γ tests. In BALB/c mice, Rv0674 adjuvant by DDA/Poly IC could also induce a high level of IFN-γ, interleukin-2 and interleukin-6 as well as a high IgG titer in both high- and low-dose groups indicating that Rv0674 is essential in humoral and cellular immunity. Moreover, the cytokine profile and IgG isotype characterized Rv0674 as a Th1/Th2-mixed-type protective immunity with the predominance of Th1 cytokines.@*CONCLUSION@#Rv0674 may be a good potential candidate for the development of TB serological diagnosis and a new TB vaccine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tuberculosis
/
Diagnosis
/
Allergy and Immunology
/
Immunity, Humoral
/
Immunity, Cellular
/
Mice, Inbred BALB C
/
Antigens, Bacterial
Type of study:
Diagnostic study
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2019
Type:
Article
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