Osthole decreases collagen I/III contents and their ratio in TGF-β1-overexpressed mouse cardiac fibroblasts through regulating the TGF-β/Smad signaling pathway / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 321-329, 2018.
Article
in En
| WPRIM
| ID: wpr-773610
Responsible library:
WPRO
ABSTRACT
The present study was designed to elucidate whether the mechanism by which osthole decreases collagenI/III contents and their ratio is regulating the TGF-β/Smad signaling pathway in TGF-β1-overexpressed mouse cardiac fibroblasts (CFs). These CFs were cultured and treated with different concentrations of osthole. Our results showed that the TGF-β1 expression in the CFs transfected with that the recombinant expression plasmids pcDNA3.1(+)-TGF-β1 was significantly enhanced. After the CFs were treated with 1.25-5 μg·mL of osthole for 24 h, the mRNA and protein expression levels of collagensIand III were reduced. The collagen I/III ratio was also reduced. The mRNA and protein expression levels of TGF-β1, TβRI, Smad2/3, P-Smad2/3, Smad4, and α-SMA were decreased, whereas the expression level of Smad7 was increased. These effects suggested that osthole could inhibit collagen I and III expression and reduce their ratio via the TGF-β/Smad signaling pathway in TGF-β1 overexpressed CFs. These effects of osthole may play beneficial roles in the prevention and treatment of myocardial fibrosis.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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RNA, Messenger
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Signal Transduction
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Cells, Cultured
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Gene Expression Regulation
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Actins
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Collagen
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Protein Serine-Threonine Kinases
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Receptors, Transforming Growth Factor beta
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Coumarins
Limits:
Animals
Language:
En
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2018
Type:
Article