The antioxidant system mediated by Nrf2 in C2C12 cells responding to HO stimulus under different oxygen concentration / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 317-321, 2019.
Article
in Zh
| WPRIM
| ID: wpr-776506
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To apply hypoxia of different oxygen concentration on C2C12 cells to study the changes of Nrf2 antioxidant system under HO.@*METHODS@#The perfect simulative effect time and concentration of HO were chosen. Cell vitality was tested after C2C12 cells cultured in 0.1 mmol/L, 0.25 mmol/L, 0.5 mmol/L, 0.75 mmol/L, 1 mmol/L and 2 mmol/L HO for 1 or 2 h respectively. The C2C12 cells were divided into different oxygen concentration group: 21%O, 12%O, 8%O, 5%O respectively. And then cells were treated with HO for 1 h, and collected for determination. Immunofluorescence of Nrf2 and the protein expression of Nrf2 were detected. The expressions of antioxidant enzymes superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), catalase(CAT), NADPH quinine oxidoreductase-1 (NQO-1), glutathione peroxidase-1 (GPX-1), Heme oxygenase-1 (HO-1) mRNA and cellular ROS levels were tested by high quality fluorescence assay.@*RESULTS@#0.5 mmol/L HO for 1 h was selected as the conditions of HOstimulation. Compared with 21% O group, the expressions of Nrf2 mRNA and protein, antioxidant enzymes SOD1, SOD2, CAT, HO-1, NQO-1, GPX-1 mRNA were increased significantly (P<0.05 or P<0.01), and ROS level was lower (P<0.01) in 12%O group cells; only the expression of GPX-1 mRNA was increased (P<0.05) in 8%O group; the expressions of Nrf2 mRNA and protein expression, antioxidant enzymes SOD1, SOD2, NQO-1, GPX-1 mRNA were decreased significantly(P<0.05 or P<0.01), and ROS level was higher (P<0.01) in 5%O group.@*CONCLUSION@#Hypoxia can affect the Nrf2 antioxidant system, and the different oxygen concentrations have different impact. In addition, 12% O for 12 h could promote the Nrf2 antioxidant system, and 5% extremely low oxygen may inhibit it.
Full text:
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Index:
WPRIM
Main subject:
Oxygen
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Cell Line
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Cell Survival
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Reactive Oxygen Species
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Oxidative Stress
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Myoblasts
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NF-E2-Related Factor 2
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Hydrogen Peroxide
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Metabolism
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Antioxidants
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Applied Physiology
Year:
2019
Type:
Article