Seroprevalence of Norwalk-Like Viruses in Seoul: Detection Using Baculovirus-Expressed Norwalk Virus Capsid Antigen
Journal of the Korean Society of Virology
; : 161-168, 1997.
Article
in Ko
| WPRIM
| ID: wpr-77983
Responsible library:
WPRO
ABSTRACT
The present study was designed to estimate the seroprevalence of NLVs among diarrheagenic children and in healthy adults in Seoul and its vicinity with the use of an EIA and an Western blot (WB) based on recombinant Norwalk virus capsid protein (rNV) and crude virus preparations as antigen. Seroconversion was observed in 34 (83%) of 41 tested using the EIA and in 21 (54%) of 39 using the WB, suggesting that the NLVs with epitopes common to rNV are prevalent in Seoul area. Diarrheal children who were known to have been infected with several other strains of the NLVs showed no significant antibody response to the rNV. Infection with rNV occurred earlier in life: primary infections with rNV were common before the age of 6 months and over 91% of children had evidence of infection by that age by the EIA. Since the amount of the NLV antigens available for seroepidemiologic surveys is limited, we tried to detect NLV antibody by using crude virus preparations as antigen. One crude virus preparation of a child whose stool yielded genetically distinct NLV revealed the presence of the plural number of bands upon SDS-PAGE, but precipitated only one band (62 kDa) after the WB with a serum (collected 10 days after the onset of symptoms) of another diarrheal child. The WB assay we present in this report revealed that the NLVs are prevalent among Korean population and that the sera contained antibody to a single major structural protein, with molecular sizes of 58 to 62 kDa, compatible with the sizes reported for the Norwalk virus and Snow Mountain agent proteins, respectively. When the results of the WB were compared with those obtained by the EIA, the EIA antibody assay was sensitive enough to detect an antibody rise of as much as 4096-fold but not as specific as the WB. The WB assay presented in this paper will provide a powerful tool to elucidate not only antigenic structures of the NLVs but also seroepidemiology of the NLV infection. The availability of an unlimited source of antigen will enable a large scale serologic studies that will greatly increase our understanding of the role of NLVs in human enteric illness.
Full text:
1
Index:
WPRIM
Main subject:
Snow
/
Seroepidemiologic Studies
/
Blotting, Western
/
Capsid
/
Norwalk virus
/
Norovirus
/
Capsid Proteins
/
Electrophoresis, Polyacrylamide Gel
/
Seoul
/
Antibody Formation
Type of study:
Diagnostic_studies
Limits:
Adult
/
Child
/
Humans
Country/Region as subject:
Asia
Language:
Ko
Journal:
Journal of the Korean Society of Virology
Year:
1997
Type:
Article