GSDME influences sensitivity of breast cancer MCF-7 cells to paclitaxel by regulating cell pyroptosis / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
; (6): 146-151, 2019.
Article
in Zh
| WPRIM
| ID: wpr-792957
Responsible library:
WPRO
ABSTRACT
@# Objective:
To investigate whether GSDME affects the sensitivity of breast cancer MCF-7 cells to paclitaxel (PTX) by regulating cell pyroptosis.Methods:
GSDME was knocked-down in MCF-7 cells by RNA interference technique. CCK-8 assay, flow cytometry,lactate dehydrogenase (LDH) release method and Wb were respectively used to detect cell proliferation, pyroptotic rate, LDH release, GSDME-N-terminal protein and cleaved-caspase-3 protein levels in PTX-treated MCF-7 cells before and after GSDME knockdown.Results:
Compared with the control group, the pyroptotic rate, LDH release, GSDME-N-terminal protein and cleaved-caspase-3 protein levels in the PTX-treatment group significantly increased (all P<0.01). Compared with the si-NC group, the PTX-sensitivity of si-GSDME group decreased, and the pyroptotic rate, LDH release and GSDME-N-terminal protein all significantly decreased (all P< 0.01).Conclusion:
Knock-down of GSDME in MCF-7 cells significantly inhibited cell pyroptosis and reduced drug sensitivity of MCF7 cells to PTX.
Full text:
1
Index:
WPRIM
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Chinese Journal of Cancer Biotherapy
Year:
2019
Type:
Article