Therapeutic Effects of Topical Application of Ozone on Acute Cutaneous Wound Healing
Journal of Korean Medical Science
;
: 368-374, 2009.
Article
in English
| WPRIM
| ID: wpr-79584
ABSTRACT
This study was undertaken to evaluate the therapeutic effects of topical ozonated olive oil on acute cutaneous wound healing in a guinea pig model and also to elucidate its therapeutic mechanism. After creating full-thickness skin wounds on the backs of guinea pigs by using a 6 mm punch biopsy, we examined the wound healing effect of topically applied ozonated olive oil (ozone group), as compared to the pure olive oil (oil group) and non-treatment (control group). The ozone group of guinea pig had a significantly smaller wound size and a residual wound area than the oil group, on days 5 (P<0.05) and 7 (P<0.01 and P<0.05) after wound surgery, respectively. Both hematoxylin-eosin staining and Masson-trichrome staining revealed an increased intensity of collagen fibers and a greater number of fibroblasts in the ozone group than that in the oil group on day 7. Immunohistochemical staining demonstrated upregulation of platelet derived growth factor (PDGF), transforming growth factor-beta (TGF-beta) and vascular endothelial growth factor (VEGF) expressions, but not fibroblast growth factor expression in the ozone group on day 7, as compared with the oil group. In conclusion, these results demonstrate that topical application of ozonated olive oil can accelerate acute cutaneous wound repair in a guinea pig in association with the increased expression of PDGF, TGF-beta, and VEGF.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ozone
/
Skin
/
Wound Healing
/
Plant Oils
/
Platelet-Derived Growth Factor
/
Acute Disease
/
Administration, Topical
/
Transforming Growth Factor beta
/
Vascular Endothelial Growth Factor A
/
Guinea Pigs
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2009
Type:
Article
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