Simultaneous detection and subtyping of porcine endogenous retroviruses proviral DNA using the dual priming oligonucleotide system
Journal of Veterinary Science
; : 269-271, 2010.
Article
in En
| WPRIM
| ID: wpr-79610
Responsible library:
WPRO
ABSTRACT
The purpose of this study was to develop a multiplex PCR that can detect porcine endogenous retrovirus (PERV) proviral genes (pol, envA, envB, envC) and porcine mitochondrial DNA, using a dual priming oligonucleotide (DPO) system. The primer specifically detected the PERV proviral genes pol, envA, envB, envC, and porcine mitochondrial DNA only in samples of pig origin. The sensitivity of the primer was demonstrated by simultaneous amplification of all 5 target genes in as little as 10 pg of pig DNA containing PERV proviral genes and mitochondrial DNA. The multiplex PCR, when applied to field samples, simultaneously and successfully amplified PERV proviral genes from liver, blood and hair root samples. Thus, the multiplex PCR developed in the current study using DPO-based primers is a rapid, sensitive and specific assay for the detection and subtyping of PERV proviral genes.
Key words
Full text:
1
Index:
WPRIM
Main subject:
DNA, Mitochondrial
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Proviruses
/
Gammaretrovirus
/
DNA Primers
/
Sus scrofa
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Journal of Veterinary Science
Year:
2010
Type:
Article