Murine enteroids culture system and Marmota Himalayana Hepatovirus replication by the enteroids / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 558-561, 2017.
Article
in Zh
| WPRIM
| ID: wpr-808833
Responsible library:
WPRO
ABSTRACT
Objective@#To establish mouse intestinal tissue and its application in the proliferation of Marmota Himalayana hepatovirus (MHHAV).@*Methods@#The intestinal crypts of Balb/C mice were digested with EDTA at a high concentration, cultured in Matrigel and passaged into frozen intestinal enteroids. The monolayers were inoculated on 96-well plates and infected with MHHAV. Real-time qPCR virus concentrations at different time points were compared with those of a number of cell lines routinely used to develop hepatitis A virus.@*Results@#The mouse intestinal tissue culture system was successfully established. MHHAV did not proliferate in the cell lines commonly used for the culture of hepatitis A virus, but proliferated well in the intestinal tissues of mice. The virus multiplied about 3 700 fold in 4 days.@*Conclusions@#Mice intestinal enteroids can be used for the isolation and culture of MHHAV.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2017
Type:
Article