The polysaccharide isolated from Pleurotus nebrodensis (PN-S) shows immune-stimulating activity in RAW264.7 macrophages / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 355-360, 2015.
Article
in En
| WPRIM
| ID: wpr-812135
Responsible library:
WPRO
ABSTRACT
A novel Pleurotus nebrodensis polysaccharide (PN-S) was purified and characterized, and its immune-stimulating activity was evaluated in RAW264.7 macrophages. PN-S induced the proliferation of RAW264.7 cells in a dose-dependent manner, as determined by the MTT assay. After exposure to PN-S, the phagocytosis of the macrophages was significantly improved, with remarkable changes in morphology being observed. Flow cytometric analysis demonstrated that PN-S promoted RAW264.7 cells to progress through S and G2/M phases. PN-S treatment enhanced the productions of interleukin-6 (IL-6), nitric oxide (NO), interferon gamma (INF-γ), and tumor necrosis factor-α (TNF-α) in the macrophages, with up-regulation of mRNA expressions of interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), interferon gamma(INF-γ) and tumor necrosis factor-α (TNF-α) being observed in a dose-dependent manner, as measured by qRT-PCR. In conclusion, these results suggest that the purified PN-S can improve immunity by activating macrophages.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
RNA, Messenger
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Cell Cycle
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Up-Regulation
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Cell Line
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Interleukin-6
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Interferon-gamma
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Tumor Necrosis Factor-alpha
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Pleurotus
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Reverse Transcriptase Polymerase Chain Reaction
Limits:
Animals
Language:
En
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2015
Type:
Article