Protective effects of shengmai san and its three fractions on cerebral ischemia-reperfusion injury / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 222-230, 2013.
Article
in En
| WPRIM
| ID: wpr-812701
Responsible library:
WPRO
ABSTRACT
AIM@#To investigate the antioxidant and anti-inflammatory effects of Shengmai San (SMS) and its ethyl acetate extract (SEa), n-butanol extract (SBu), and aqueous extract (SWe), and clarify the material base of SMS and the roles played by its fractions.@*METHODS@#A mouse model of transient forebrain ischemia/reperfusion (I/R) by means of common carotid artery occlusion (CCAO) was used to investigate the effects of SMS and its three fractions. Histopathological damage, blood-brain barrier disruption, and antioxidant and inflammation-related parameters, including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), myeloperoxidase (MPO), nitric oxide (NO), tumor necrosis factor-α (TNF-α) were measured. The chemical constituents of each fraction were identified by LC-MS.@*RESULTS@#Eighteen lignans in SEa, and thirteen steroidal glycosides and ginsenosides in SBu were determined. SMS significantly inhibited I/R induced formation of histological injury and cerebral MPO activity. SMS showed the strongest antioxidant and anti-inflammatory effects against the I/R-caused injuries. SEa showed higher antioxidant activity than the other two fractions and SBu has a slightly stronger inhibition on the productions of NO and TNF-α.@*CONCLUSION@#SMS as a whole had the most effective protection against cerebral I/R-caused injuries compared with its fractions, which inferred that it contains different groups of compounds that contribute together to its protective effect.
Full text:
1
Index:
WPRIM
Main subject:
Superoxide Dismutase
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Drugs, Chinese Herbal
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Reperfusion Injury
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Chemistry
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Chromatography, High Pressure Liquid
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Nitric Acid
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Peroxidase
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Oxidative Stress
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Protective Agents
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Disease Models, Animal
Type of study:
Prognostic_studies
Limits:
Animals
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Humans
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Male
Language:
En
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2013
Type:
Article