Application value of frozen-thawed mouse round spermatids in in vitro fertilization / 中华男科学杂志
National Journal of Andrology
; (12): 1059-1062, 2017.
Article
in Zh
| WPRIM
| ID: wpr-812835
Responsible library:
WPRO
ABSTRACT
Objective@#To investigate the application value of the frozen-thawed round spermatids of the mouse in in vitro fertilization (IVF).@*METHODS@#Haploid spermatids of the mouse obtained in vitro were divided into a frozen-thawed and a fresh group and oocytes were collected from 6-8 weeks old female mice. After diamidino-phenyl-indole (DAPI) staining, the oocytes were subjected to intracytoplasmic round spermatid injection (ROSI), 259 in the frozen-thawed and 238 in the fresh group. Comparisons were made between the two groups in the capacities of fertilization and embryonic development.@*RESULTS@#The survival rate of the frozen-thawed haploid spermatids was (75.9 ± 2.3) %. No statistically significant differences were observed between the frozen-thawed and fresh groups after ROSI in the rates of fertilization (51.9 vs 55.7%, P >0.05), 2-cell embryos (51.0 vs 62.2%, P >0.05), 4-8-cell embryos (41.8 vs 42.9%, P >0.05), or morula-blastocysts (12.2 vs 21.4%, P >0.05).@*CONCLUSIONS@#Frozen-thawed round spermatids of the mouse are similar to fresh ones in their capacities of fertilization and embryonic development.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Oocytes
/
Spermatids
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Transplantation
/
Fertilization in Vitro
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Cryopreservation
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Sperm Injections, Intracytoplasmic
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Embryonic Development
Limits:
Animals
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Pregnancy
Language:
Zh
Journal:
National Journal of Andrology
Year:
2017
Type:
Article