Establishment and preliminary mechanism study of the zebrafish strain of KIAA0196: A candidate pathogenic gene for heart development / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 968-975, 2019.
Article
in Zh
| WPRIM
| ID: wpr-813062
Responsible library:
WPRO
ABSTRACT
To explore the effects of KIAA0196 gene on cardiac development and the establishment of zebrafish strain.
Methods: Peripheral blood and gDNA from patients were extracted. Copy number variation analysis and target sequencing were conducted to screen candidate genes. The KIAA0196 knockout zebrafish was generated by CRISPR/Cas9 to detect whether KIAA0196 deficiency could affect cardiac development. Finally, the wild-type and mutant zebrafish were anatomized and histologically stained to observe the phenotype of heart defects.
Results: The KIAA0196 knockout zebrafish strain was successfully constructed using CRISPR/Cas9 technology. After 60 hours fertilization, microscopic examination of KIAA0196 knockout zebrafish (heterozygote + homozygote) showed pericardial effusion, cardiac compression and severely curly tail. Compared with wild-type zebrafish, the hearts of mutant KIAA0196 zebrafish had cardiac defects including smaller atrium and larger ventricle, and the myocardial cells were looser.
Conclusion: KIAA0196 gene plays an important regulatory role in the development of heart. It might be a candidate gene for congenital heart disease.
Full text:
1
Index:
WPRIM
Main subject:
Phenotype
/
Zebrafish
/
Proteins
/
Zebrafish Proteins
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Myocytes, Cardiac
/
DNA Copy Number Variations
/
Genetics
/
Heart
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Heart Defects, Congenital
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2019
Type:
Article