Construction of two hepatocellular carcinoma cell models for the expression of HBV X gene with different selection characteristics / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 636-639, 2005.
Article
in Zh
| WPRIM
| ID: wpr-813459
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To construct 2 hepatocellular carcinoma (HCC) cell models for the expression of HBV X gene with different selection characteristics.@*METHODS@#HepG2 HCC cells were infected with eukaryotic expression vectors with HBV X gene, pCEP4-X, and pcDNA3. 1 (+)-X. Single cell clone was selected by hygromycin and neomycin. After propagating culture for certain periods, the HBV X gene expression was identified by PCR, RT-PCR, and Western blot.@*RESULTS@#Single HCC cell clone with HBV X gene transferred resistant to hygromycin and neomycin was selectively cultured, and the cells could be propagated for certain periods. PCR, RT-PCR, and Western blot identified the expression of HBV X gene.@*CONCLUSION@#Two HCC cell models for the expression of HBV X gene with different selection characteristics have been successfully constructed.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Trans-Activators
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Carcinoma, Hepatocellular
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Cell Line, Tumor
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Eukaryotic Cells
/
Genetic Vectors
/
Genetics
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Liver Neoplasms
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Metabolism
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Models, Biological
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2005
Type:
Article