Expression of hCTLA4-Ig mediated by adeno-associated virus in porcine islets and their biological activity / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 36-40, 2007.
Article
in Zh
| WPRIM
| ID: wpr-813940
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To evaluate the expression of hCTLA4-Ig and their biological function in newborn porcine islets (NPIs) transfected with AAV-hCTLA4-Ig.@*METHODS@#Cultured NPIs were transfected with AAV-hCTLA4-Ig. The expression of CTLA4-Ig in these NPIs was assayed by RT-PCR and immunocytochemistry. The levels of IL-2, IFN-gamma, and TNF-alpha in the culture medium were assayed by ELISA after these cells the co-cultured with human. The response of glucose-stimulated insulin secretion was observed in the transgene group and the control group.@*RESULTS@#The expressions of CTLA4-Ig mRNA and protein were detected in the transgene group. The levels of cytokines were obviously lower in the transgene group than those in the control group (P0.05).@*CONCLUSION@#AAV mediated hCTLA4-Ig expression in NPIs could inhibit T lymphocyte to produce cytokines, while the endocrine functions of the NPIs were not significantly affected.
Full text:
1
Index:
WPRIM
Main subject:
Swine
/
Recombinant Fusion Proteins
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Immunoglobulin Fc Fragments
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Enzyme-Linked Immunosorbent Assay
/
Immunohistochemistry
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Transfection
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Antigens, Differentiation
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Antigens, CD
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Gene Expression
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Cells, Cultured
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2007
Type:
Article