Proteomic analysis of Helicobacter pylori in human gastritis and gastric cancer / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 384-390, 2008.
Article
in Zh
| WPRIM
| ID: wpr-814067
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To establish 2-dimensional electrophoresis (2-DE) maps of Helicobac-ter pylori in human gastritis, and gastric cancer, to identify the differentially expressed proteins,and to discuss the role of bacterial factor in pathogenesis.@*METHODS@#The total proteins of Helicobacter pylori in human gastritis and gastric cancer were separated by immobilized pH gradient-based 2-DE. The differentially expressed proteins were screened by PDQuest analysis software and identified by peptide mass fingerprint based on matrix-assisted laser desorption/ionization time of flight mass spectrometry, and searched on database.@*RESULTS@#A well-resolved and reproducible 2-DE pattern of Helicobacter pylori was obtained from patients with human gastritis and gastric cancer. Fourteen differentially expressed proteins were identified, including proteins related to anti-oxidation,molecular chape-rones and detoxification, enzymes related to metabolism,proteins related to cytoarchitecture,and proteins related to signal conduction.@*CONCLUSION@#A well-resolved and reproducible 2-DE pattern of Helicobacter pylori in human gastritis and gastric cancer is established and differentially expressed proteins from these 2 diseases are identified. The differentiation of protein expression may play an important role in the pathogenesis of gastric cancer.
Full text:
1
Index:
WPRIM
Main subject:
Stomach Neoplasms
/
Bacterial Proteins
/
Electrophoresis, Gel, Two-Dimensional
/
Helicobacter pylori
/
Helicobacter Infections
/
Proteome
/
Proteomics
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Gastritis
/
Methods
/
Microbiology
Type of study:
Prognostic_studies
Limits:
Female
/
Humans
/
Male
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2008
Type:
Article